AustralieFrV1, Main, Exploration, bibRecord, 00AE51

RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae

Identifieur interne : 00AE51 ( Main/Exploration ); précédent : 00AE50; suivant : 00AE52

RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae

Auteurs : Saengchan Senapin ; G. Desmond Clark-Walker ; Xin Jie Chen ; Bertrand Séraphin ; Marie-Claire Daugeron

Source :

Nucleic Acids Research [ 0305-1048 ] ; 2003.

RBID : PMC:156047

Abstract

A strain of Saccharomyces cerevisiae, defective in small subunit ribosomal RNA processing, has a mutation in YOR145c ORF that converts Gly235 to Asp. Yor145c is a nucleolar protein required for cell viability and has been reported recently to be present in 90S pre-ribosomal particles. The Gly235Asp mutation in YOR145c is found in a KH-type RNA-binding domain and causes a marked deficiency in 18S rRNA production. Detailed studies by northern blotting and primer extension analyses show that the mutant strain impairs the early pre-rRNA processing cleavage essentially at sites A₁ and A₂, leading to accumulation of a 22S dead-end processing product that is found in only a few rRNA processing mutants. Furthermore, U3, U14, snR10 and snR30 snoRNAs, involved in early pre-rRNA cleavages, are not destabilized by the YOR145c mutation. As the protein encoded by YOR145c is found in pre-ribosomal particles and the mutant strain is defective in ribosomal RNA processing, we have renamed it as RRP20.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC156047

PubMed: 12736301
PubMed Central: 156047

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>A strain of <italic>Saccharomyces cerevisiae</italic>
, defective in small subunit ribosomal RNA processing, has a mutation in <italic>YOR145c</italic>
 ORF that converts Gly235 to Asp. Yor145c is a nucleolar protein required for cell viability and has been reported recently to be present in 90S pre-ribosomal particles. The Gly235Asp mutation in <italic>YOR145c</italic>
 is found in a KH-type RNA-binding domain and causes a marked deficiency in 18S rRNA production. Detailed studies by northern blotting and primer extension analyses show that the mutant strain impairs the early pre-rRNA processing cleavage essentially at sites A<sub>1</sub>
 and A<sub>2</sub>
, leading to accumulation of a 22S dead-end processing product that is found in only a few rRNA processing mutants. Furthermore, U3, U14, snR10 and snR30 snoRNAs, involved in early pre-rRNA cleavages, are not destabilized by the <italic>YOR145c</italic>
 mutation. As the protein encoded by <italic>YOR145c</italic>
 is found in pre-ribosomal particles and the mutant strain is defective in ribosomal RNA processing, we have renamed it as <italic>RRP20</italic>
.</p>
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Serveur d'exploration sur les relations entre la France et l'Australie

RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae

RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae

Source :

Abstract

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